M.D.: Department of Clinical Medicine. Jining Medical College, China, 2001.
M.Sc.: Department of Pathology, Shanghai Medical College, Fudan University, China, 2004.
Ph.D.: Department of Microbiology and Immunology, Medical College of Wisconsin, 2010.
Lab Phone: 225-578-5196
Office: A606 Life Sciences Annex
Area of Interest:
Host-Pathogen interactions, Innate immunity, Bacterial toxins, Glycobiology.
The main goal of my laboratory is to understand how S. aureus seamlessly transitions between infection and colonization. Although S. aureus colonization (30% of humans) is strongly associated with higher risks for clinical infections, most colonized individuals will not experience S. aureus infections, suggesting that S. aureus maintains a dedicated balance with the host.
There are three areas of focus in the laboratory:
- To understand the molecular and cellular interactions between bacterial pathogens and hosts innate immune system, especially bacterial toxins and secreted proteins and their neutrophil manipulation activities.
- To understand the contribution of glycans for bacterial pathogenesis.
- To develop therapeutic and diagnostic platforms, using bacterial toxins and secreted proteins against human diseases.
I invite graduate and undergraduate students with an interest in my research to contact me.
Yang C, Dahms N, Barbieri JT, Chen C. Multiple Domains of Staphylococcal Superantigen-like Protein 11 (SSL11) Contribute to Neutrophil Inhibition. Biochemistry, 2022, 61(7): 616-624.
Chen, C., Yang, C. & Barbieri, J. T. Staphylococcal Superantigen-like protein 11 mediates neutrophil adhesion and motility arrest, a unique bacterial toxin action. Sci Rep 9, 4211, doi:10.1038/s41598-019-40817-x (2019).
Chen C and Barbieri JT. Detection of ADP-Ribosylating Bacterial Toxins (Book Chapter 20). Methods Mol Biol. 1813:287-295, 2018.
Pellett S, Bradshaw M, Tepp WH, Pier CL, Whitemarsh RCM, Chen C, Barbieri JT, Johnson EA. The Light Chain Defines the Duration of Action of Botulinum Toxin Serotype A Subtypes. mBio, 9(2), 00089-18, 2018
Chen C, Barbieri JT. When Escherichia coli doesn’t fit the mold: A pertussis-like toxin with altered specificity. J Biol Chem, 292(36), 15159-15160, 2017.
Chen C, Przedpelski A, Tepp WH, Pellett S, Johnson EA, Barbieri JT. Heat-Labile Enterotoxin IIa, a Platform To Deliver Heterologous Proteins into Neurons. mBio, 6, 00734-15, 2015.
Zuverink M, Chen C, Przedpelski A, Blum FC, Barbieri JT. A heterologous Reporter defines the Role of the Tetanus Toxin Interchain Disulfide in Light-Chain. Infect Immun. 83, 2714-24, 2015.
Yang C, Chen C, Sorokin A. Prostaglandin E2 modifies SMAD2 and promotes SMAD2-SMAD4 complex formation. Prostaglandins Leukot Essent Fatty Acids. 90 145-9, 2014.
Chen C, Krishnan V, Macon K, Manne K, Narayana SV, Schneewind O. Secreted proteases control autolysin-mediated biofilm growth of Staphylococcus aureus. Journal of Biological Chemistry, 288 29440-52, 2013.
Blum FC, Chen C, Kroken AR, Barbieri JT. Tetanus toxin and botulinum toxin a utilize unique mechanisms to enter neurons of the central nervous system. Infect Immun. 80,1662-9, 2012.
Pier CL, Chen C, Tepp WH, Lin G, Janda KD, Barbieri JT, Pellett S, Johnson EA. Botulinum neurotoxin subtype A2 enters neuronal cells faster than subtype A1. FEBS Lett. 585,199-206, 2011.
Chen, C., Fu Z., Kim J.J., Barbieri, J. T. and Baldwin, M. R. Gangliosides as high affinity receptors for tetanus neurotoxin. Journal of Biological Chemistry, 284, 26569-577, 2009.
Fu Z., Chen C., Barbieri, J. T., Kim J.J., and Baldwin, M. R. Glycosylated SV2 and gangliosides as dual receptors for botulinum neurotoxin serotype F. Biochemistry, 48, 5631-41, 2009.
Chen C., Baldwin, M. R., and Barbieri, J. T. Molecular basis for tetanus toxin coreceptor interactions. Biochemistry, 47, 7179-86, 2008.
Chen C., Zhang J, Li J, Huang J, Yang C, Huang G, Shi J. Hydrodynamic-based in vivo transfection of retinoic X receptor- gene can enhance vitamin A-induced attenuation of liver fibrosis in mice. Liver International. 24: 679-686, 2004.