Brightfield & Fluorescence Microscopy

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Leica DM6B with Deconvolution Software

The Leica DM6 B upright fluorescent microscope is equipped with Hamamatsu sCMOS camera, Leica DFC450 color CCD, and LAS X extended software package.

Leica DM6B

Leica DM6B in Chemistry & Materials Building, Room 155.

  • Differential Interference Contrast
  • Polarized Imaging

Metal halide lamp and filter sets:

  • DAPI/UV: Ex 325-375/Em 435-485
  • GFP: Ex 450-490/Em 500-550
  • TRITC: Ex 532-558/Em 570-640
  • TXR: Ex 540-580/Em 592-668
  • Cy5: Ex 590-650/Em 662-738
  • Cy7: Ex 672-747/Em 765-855

Quad Sedat EFW:

  • DAPI/UV: Ex 325-375/Em 430-480
  • FITC: Ex 480-500/Em 507-543
  • TRITC: Ex 542-568/Em 579-631
  • Cy5: Ex 630-660/Em 669-741

Fura:

  • Fura2-ET EFW: Ex 340 and 380/Em 470-550

Fret Set:

  • CFP: Ex 422-432/Em 465-495
  • YFP: Ex 498-510/Em 529-566

  • Dry: 5x/0.15, 10x/0.30, 20x/0.80, 40x/0.85
  • Water immersion: 20x/0.75, 40x/0.80
  • Oil immersion: 40x/1.25-0.75, 40x/1.30, 63x/1.40-0.60, 63x/1.40, 100x/1.4-0.70, 100x/1.30

  • DFC450
  • Hamamatsu Flash4 CL

  • Multichannel acquisition and image overlay with labeling
  • Constrained iterative deconvolution in 2-D and 3-D
  • 3-D reconstruction (surface and volume)
  • Time lapse
  • Montage image
  • Analysis, graphs and spreadsheets of data
  • Resolution of camera 2048 x 2048 pixel
  • 100 frames per second (fps) at full resolution in Standard Scan mode or 30 fps in Slow Scan mode

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Leica Thunder Model Organism Stereomicroscope 

Leica MC205FA stereo/macroscope.  Fully automated Z drive with 420 mm range and 1 µm z-resolution.  Manual zoom from 0.78 to 16X.  Four position filter turret; 2 coded filter sets. High precision scanning stage with integrated controller; position resolution <= 5 nm and maximum load capacity of 1.5 kg. 

Leica Thunder

Leica Thunder Model Organism Stereomicroscope in Chemistry & Materials Building, Room 155.

  • ET GFP Ex 450-490/Em 500-550
  • ET mCHER – Ex 540-580/Em 593-667

  • PLANAPO 1.0.x, working distance 61.5mm
  • PLANAPO 5.0x/0.50 LWD working distance 19mm

  • Leica TL5000 Ergo Transmitted Light Base
  • Leica LED5000 RL-80/40
  • Leica LED3 (fluorescence), wavelength white 390-680 nm

  • Multichannel fluorescence acquisition
  • Z-stack capture
  • Time lapse
  • Measurement and Data Analysis

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