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Marker-Aided Development and
Characterization of an Introgression Library to Discover
Alleles for Weediness: Red Rice as a Model System
INVESTIGATOR: Subudhi, P. K.; Cohn, M. A.
PERFORMING INSTITUTION:
AGRONOMY
LOUISIANA STATE UNIVERSITY
BATON ROUGE, LOUISIANA 70893
NON-TECHNICAL SUMMARY: Limited knowledge of the
origins and evolution of weedy characteristics of monocots
limits the formulation of novel genetic strategies to
improve crop productivity. Red rice, a noxious weed in rice
growing areas of Southern United States, will be used as a
model to elucidate the molecular basis of weediness, with
special emphasis on seed dormancy and seed shattering, which
are two key traits for survival and persistence of the
majority of weed species. Besides advancing the field of
weed biology through genetic analysis of above weedy
characteristics, this project will have great practical
utility to analyze other traits of agronomic importance to
improve rice productivity in the United States.
OBJECTIVES: Develop an introgression library of red
rice genomic fragments in a cultivated rice background.
Characterize the genetic make-up of the introgression
library. Phenotypically characterize the introgression
library. Localize the genes or quantitative trait loci (QTL)
for the key weedy attributes, such as seed shattering and
seed dormancy onto the defined introgressions segments.
APPROACH: A set of unique introgression lines with
fragments of the red rice genome in a cultivated rice
background will be developed to elucidate the molecular
basis of weedy characteristics. Molecular markers will be
exploited to track the red rice chromosome segments and the
recovery of the recurrent parental genome during three
successive backcross generations to facilitate quick and
successful development of an introgression library. Finally,
the introgression lines will be phenotypically and
genotypically characterized to link the introgressed
fragments to complex weedy attributes such as seed dormancy
and seed shattering.
PROJECT CONTACT:
Name: Subudhi, P. K.
Phone: 225-578-1303
Fax: 225-578-1403
Email:
psubudhi@agctr.lsu.edu
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