PhD, Osmania University
MSc, Osmania University
BSc, Osmania University
My primary research interests are focused on the development and testing of Oncolytic Herpes Viral Vaccines in the treatment of Melanoma. We have developed an invivo model for subcutaneous xenografts of melanoma cell lines in BalbC mice. These tumors will be treated with the Oncolytic Virus OnCSynD expressing PGDH. The effect of oncolytic viral therapy on tumor growth, angiogenesis and metastasis is currently being studied.
Currently I am also working on using waste water sampling as a predictive tool for determining COVID-19 outbreaks. Studies have shown an 8-day temporal shift between covid-19 detection in waste water samples and hospital consultations for COVID-19.
I am also the lead researcher studying the role of Kaposi’s Sarcoma associated Herpes Virus (KSHV) envelope glycoproteins in the regulation of tumor related angiogenesis using shRNA based silencing. that regulating the expression of certain glycoproteins can alter the ability of KSHV infected cells to induce formation of tumor associated vasculature. Using Q-PCR and an angiogenesis microarray I was able to isolate potential pathway triggers associated with induction of angiogenesis associated with KHSV glycoproteins, consequently identifying viable therapeutic targets in KS tumors. Since a number of KSHV glycoproteins are conserved across the herpes viruses, these therapeutics can have broad range applications. I am actively involved in the use of the ion torrent personal genome machine for whole genome sequencing and RNAseq of small organisms. With recent addition of the Ion Proton, I have been involved in RNAseq analysis of human cell lines. I have also undergone training for use of the Quant Studio Flex RealTime PCR system, which we use for rare event detection. Targeted downregulation of viral gene expression using shRNAs combined with whole transcriptome analysis by NextGen sequencing will provide valuable insight into global cellular response to viral infection as well as identify therapeutic targets for virus induced cancer treatment.
In addition to my primary research project where I study, the regulation of angiogenesis in Kaposi’s sarcoma and tumors in general, I am also involved in providing technical guidance and support in Molecular biology to NIH centers associated with LSU. I have cloned multiple genes, and expressed functional protein from novel sources which were in many cases done for the first time in those organisms. I have also worked closely with these labs to design functional assays to assess protein function. Additionally, I have collaborated with my colleagues to develop a protocol for establishment of Ganglionic axons in vitro for visualization of HSV-1 entry into axons.
I have been teaching graduate level -Ology course on Viruses and Cancers to SVM Grad students for the past five years as well as conducting laboratory training. During those teaching sessions I have always felt that one of the strong points of my teaching style was the ability to connect with my wards on a one-on-one basis and convey my enthusiasm for the subject through my lectures and interactions. It has been my experience that the best ways to infuse enthusiasm for a subject in students, is by instilling a sense of discovery, and never forget that, learning is a continuous process for both teacher and ward. I have always shared my presentations that include my notes so the students know what I am thinking in relation to the subject discussed.
Factors Affecting Growth Kinetics and Spontaneous Metastasis in the B16F10 Syngeneic Murine Melanoma Model., Fowlkes, N; Clemons, K; Rider, Paul; Subramanian, Ramesh; Wakamatsu, Nobuko; Langohr, Ingeborg; Kousoulas, Konstantin, 2019, Comparative medicine , Volume: 69, Number: 1, Pages: 48-54
A single intramuscular vaccination of mice with the HSV-1 VC2 virus with mutations in the glycoprotein K and the membrane protein UL20 confers full protection against lethal intravaginal challenge with virulent HSV-1 and HSV-2 strains., Stanfield, Brent; Stahl, J; Chouljenko, Vladimir; Subramanian, Ramesh; Charles, A; Saied, A; Walker, J; Kousoulas, Konstantin, 2014, PloS one , Volume: 9, Number: 10, Pages: e109890
Center for Pre-Clinical Disease Research , National Institutes of Health - NIH, $11,192,691.00