SPRING SEMINAR SERIES                       

Comparative Biomedical Sciences                  

School of Veterinary Medicine

Room 1212C - 12 Noon

 

May 17, 2018

Adita Das, PhD

Assistant Professor

Department of Comparative Biosciences

College of Veterinary Medicine

University of Illinois at Urbana-Champaign

 

Host: Dr. Levent Dirikolu

 

Anti-inflammatory Bioactive Lipids from the Cytochrome

P4501 epoxygenase Pathway

 

The human body contains endocannabinoids that elicit similar psychoactive and anti-nociceptive effects to phytocannabinoids in cannabis. Herein we report on the endogenous production of a previously unknown class of ω-3 PUFA–derived endocannabinoid epoxides that originate from the crosstalk between endocannabinoid and cytochrome P450 (CYP) epoxygenase metabolic pathways. The ω-3 endocannabinoid epoxides (eCB epoxides) are derived from docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA). These eCB epoxides are endogenously present in rat brain and peripheral organs as determined via targeted lipidomics methods. These metabolites were directly produced by activated BV-2 microglial cells via the cytochrome P450 epoxygenases. Neuroinflammation studies revealed that the eCB epoxides dose-dependently abated proinflammatory cytokines while increasing anti-inflammatory cytokines, in part through activating cannabinoid receptor-2 (CB2) and PPAR gamma. Furthermore, the ω-3 eCB epoxides exerted antiangiogenic effects in human microvascular endothelial cells (HMVEC) and vasodilatory actions on bovine coronary arteries and reciprocally regulated platelet aggregation in washed human platelets. Taken together, the ω-3 eCB epoxides’ physiological effects are mediated through both endocannabinoid and epoxyeicosanoid signaling pathways. Furthermore, we examined the anti-inflammatory and anti-apoptotic role of the six different regioisomers of eCB epoxides that showed wide range of activity towards cannabinoid receptors 1 & 2. In a separate study, we show that phytocannabinoids in cannabis inhibit metabolism of endocannabinoids by CYPs.  

 

 

Thanks to our Guest Speakers for Spring 2018

 

January 11, 2018

Thank you, Natalie M. Johnson!

Professor of Immunology & Microbiology
Department of Environmental & Occupational Health
Texas A&M University School of Public Health

 

January 25, 2018

Thank you, Ronald Klein!

Professor
Department of Pharmacology, Toxicology & Neuroscience
Louisiana State University Health Sciences Center-Shreveport

 

February 8, 2018

Thank you, Michael F. Salvatore!

Associate Professor
Institute for Healthy Aging, Center for Neuroscience Discovery
University of North Texas Health Science Center

 

February 22, 2018

Thank you, Judith T. Zelikoff!

Professor
Laboratory of Pulmonary & Systemic Toxicology
New York University School of Medicine

 

March 8, 2018

Thank you, Karen P. Maruska!

Assistant Professor
Department of Biological Sciences
Louisiana State University

 

March 22, 2018

Thank you, Mimi Sammarco!

Assistant Professor
Department of Surgery
Tulane University, New Orleans

 

April 5, 2018

Thank you, Jessy Satyadas Deshane!

Associate Professor
Medicine/Division of Pulmonary, Allergy
& Critical Care Medicine
University of Alabama - Birmingham

 

April 19, 2018

Thank you, Claudia Jakubzick!

Associate Professor
Department of Pediatrics, National Jewish Health
Department of Microbiology and Immunology
University of Colorado School of Medicine

 

May 3, 2018

Thank you, James Luyendyk!

Associate Professor
Pathology and Diagnostic Investigation
Food Safety Toxicology
Michigan State University