Area of Interest
Research areas include "Glycobiology", the study of the biological function of complex saccharides, including structural characterization using mass spectrometry and other methods. Cell surface carbohydrates of microbes and eucaryotes are studied with a focus on use of these saccharides for detection by analyte specific probes such as antibodies, lectins and enzymes. Microbial diagnostics, Fungal diagnostics using catalytically disabled endoglycosidase binding assays. Chitin and chitosan enzymology and chemistry. Biochemistry of the inflammatory process.
Current emphasis includes research on several aspects of Formosan Subterranean Termite, including the defense gland components, a novel lysozyme-protease inhibitor protein from the defense fluid and other proteins that resemble lipid-carrying proteins. We also work on analysis of juvenile hormone and other components of the defense fluid, including ceramides containing novel sphingosines. We work on termite and insect-active sesquiterpenes and other natural insect repellants and toxicants, feeding attractants and trail following molecules. Recently we have had extensive research on the effects of rare sugars on the termites and their obligate gut protozoans."
Guo, C, Ghadersohi, S, Kephart, GM, Laine, RA, Sherris, DA, Kita, H, Ponikau, JU, 2012, “Improving the Detection of Fungi in Eosinophilic Mucin: Seeing What We Couldn’t See Before”, Otolaryngology–Head and Neck Surgery 147: 943-949. http://www.ncbi.nlm.nih.gov/pubmed/22687329.
Laine, RA, Unlap, T, 2012, “IPX-750, A Dopamine Gluconamine That Binds D1/D5 Receptors and Has Anti-Parkinsonian Effects in Three Animal Models, is Transported across the Blood Brain Barrier", Journal of Biotechnology and Biomaterials 2: 5 (2012) ISSN:2155-952X, http://dx.doi.org/10.4172/2155-952X.1000142
Rosu C, Negulescu I, Cueto R, Laine RA, Daly, WH, 2013, “Synthesis and Characterization of Complex Mixtures Consisting of Cyclic and Linear Polyamides from Ethyl Bis-Ketal Galactarates” J. Macromolecular Sci, Part A, 50: 940-952 DOI: 10.1080/10601325.2013.813795 ISSN:1060-1325
Veillon L, Bourgeois J, 1, LeBlanc A, Henderson G, Marx B, Muniruzzaman S, Laine, RA, 2014, “Myo-Inositol and Phytate are Toxic to Formosan Subterranean Termites (Isoptera: Rhinotermitidae), J. of Economic Entomology 107: 1800–1812.
Xue, J., Laine, RA, Matta, K (2014), (epublished online 8/2014) “Enhancing Mass Spectrometry Strategy for Carbohydrate Analysis: Constructing a "B2 Ions" Library as an aid for Novel Relational Search Routines in MSn Analysis” Journal of Proteomics 112: 224-249. http://www.sciencedirect.com/science/article/pii/S1874391914003741
Horia Negulescu, Youzhong Guo, Thomas P. Garner, Octavia Y. Goodwin, Gregg Henderson, Roger A. Laine, and Megan A. Macnaughtan, 2015, “A Kazal-type serine protease inhibitor from the defense gland secretion of the subterranean termite Coptotermes formosanus Shiraki” PLOS1 10(5): e0125376. DOI: 10.1371/journal.pone.0125376
Zhaorigetu Chen, Roger A. Laine, 2015, Fatty Acids Differ Significantly in Castes of the Formosan Subterranean Termite (Isoptera: Rhinotermitidae) Annals of the Entomological Society of America; doi: 10.1093/aesa/sav117
Rosu, Cornelia , Paul S. Russo, William H. Daly, Rafael Cueto, John A. Pople, Roger A. Laine, and Ioan I. Negulescu (2015) “Sugar-Based Polyamides: Self-Organization in Strong Polar Organic Solvents” Biomacromolecules Article ASAP DOI: 10.1021/acs.biomac.5b00977
Zheng, J, Wang, M, Wei, W, Keller, JN, Adhikari, B, King, JF, King, ML, Peng, N, and Laine, RA (2016) “Dietary plant lectins appear to be transported from the gut to gain access to and alter dopaminergic neurons of Caenorhabditis elegans, a potential etiology of Parkinson’s disease” Frontiers in Nutrition, - Neuroenergetics and Brain Health 3: 1-16 (doi: 10.3389/fnut.2016.00007)
Zheng J, Gao C, Wang M, Tran P, Mai N, Finley JW, Heymsfield SB, Greenway FL, Li Z, Heber D, Burton JH, Johnson WD & Laine RA (2016): “Lower Doses of Fructose Extend Lifespan in Caenorhabditis elegans”, Journal of Dietary Supplements 14: 264-277, DOI: 10.1080/19390211.2016.1212959
Rosu, Cornelia; Cueto, Rafael; Veillon, Lucas; Laine, Roger; David, Connie; Russo, Paul, (2016), "Discovering Volatile Chemicals from Window Weatherstripping through Solid Phase Microextraction Gas Chromatography/Mass Spectroscopy" The Journal of Chemical Education, Manuscript ID: DOI 10.1021/acs.jchemed.6b00791 http://pubs.acs.org/articlesonrequest/AOR-ESEpNa4DgX9V8GQIITJQ
Grimball B, Veillon L, Calhoun T, Fronczek FR, Arceneaux E, and Laine, RA (2017) Cyclohexylamine Inexplicably Induces Antennae Loss in Formosan Subterranean Termites (Coptotermes formosanus Shiraki): Cyclohexylamine Hydrogen Phosphate Salts are Novel Termiticides, Pest Management Science 73: 2039-2047 ((wileyonlinelibrary.com) DOI 10.1002/ps.4610).
Namrata Karki N, Sita Aggarwal S, Laine RA, Greenway F, Losso JN, (2017) Juglone modulates intracellular level of reactive oxygen species and suppresses pancreatic cancer cell proliferation by inhibiting the KRAS-driven signaling pathways. Phytomedicine (in review)
Namrata Karki, Sita Aggarwal, Frank Greenway, Roger A. Laine, Jack N. Losso, (2017) Juglone down-regulates the Akt-HIF- 1α and VEGF signaling pathways and inhibits angiogenesis in pancreatic cancer in vitro. (Phytomedicine (in review)
M. Miyazaki,T. Nishimura,W. Hojo,T. Miyazaki,R.A. Laine,T. Yamashita (2017) Potential use of domestic cat (Felis catus) urinary extracts for manipulating the behavior of free-roaming cats and wild small felids. Applied Animal Behaviour Science 196, 52-60. https://doi.org/10.1016/j.applanim.2017.07.003 available online.
Patents (out of 26 total):
• Henderson, G, Zhu, B.C-R., Laine, Roger A., 2005, “Extracts of Vetiver Oil as Termite Repellent and Toxicant, US Patent #6890960.
• Zhu, BCR, G Henderson and RA Laine, 2005, "Dihydronootkatone and Tetrahydronootkatone as Repellents to Arthropods, US Patent #6897244.
• Sauer, A, Crowe, W, Laine, RA, Henderson, G, 2006, Efficient and economic asymmetric synthesis of nootkatone, tetrahydronootkatone, their precursors and derivatives, US Patent #7,112,700.
• Henderson, G, SA Ibrahim, R. Patton, R.A. Laine, BCR Zhu and F Chen, (2008) "Naphthalene Derivatives as Termite Repellents and Toxicants” US Patent #7351744.
• Laine, RA, Veillon, L., Muniruzzaman, S, Calhoun, T, "Rare Sugars as Toxicants for Subterranean termites", (Submitted 2009)
• Laine, RA, Veillon, L, Henderson, G., 2017, “Cyclohexylamine-based Compounds and Uses Thereof, US Patent Application Serial number 62/309,143