SPRING SEMINAR SERIES
Comparative Biomedical Sciences
School of Veterinary Medicine
Room 1212C - 12 Noon
May 17, 2018
Adita Das, PhD
Department of Comparative Biosciences
College of Veterinary Medicine
University of Illinois at Urbana-Champaign
Host: Dr. Levent Dirikolu
Anti-inflammatory Bioactive Lipids from the Cytochrome
P4501 epoxygenase Pathway
The human body contains endocannabinoids that elicit similar psychoactive and anti-nociceptive effects to phytocannabinoids in cannabis. Herein we report on the endogenous production of a previously unknown class of ω-3 PUFA–derived endocannabinoid epoxides that originate from the crosstalk between endocannabinoid and cytochrome P450 (CYP) epoxygenase metabolic pathways. The ω-3 endocannabinoid epoxides (eCB epoxides) are derived from docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA). These eCB epoxides are endogenously present in rat brain and peripheral organs as determined via targeted lipidomics methods. These metabolites were directly produced by activated BV-2 microglial cells via the cytochrome P450 epoxygenases. Neuroinflammation studies revealed that the eCB epoxides dose-dependently abated proinflammatory cytokines while increasing anti-inflammatory cytokines, in part through activating cannabinoid receptor-2 (CB2) and PPAR gamma. Furthermore, the ω-3 eCB epoxides exerted antiangiogenic effects in human microvascular endothelial cells (HMVEC) and vasodilatory actions on bovine coronary arteries and reciprocally regulated platelet aggregation in washed human platelets. Taken together, the ω-3 eCB epoxides’ physiological effects are mediated through both endocannabinoid and epoxyeicosanoid signaling pathways. Furthermore, we examined the anti-inflammatory and anti-apoptotic role of the six different regioisomers of eCB epoxides that showed wide range of activity towards cannabinoid receptors 1 & 2. In a separate study, we show that phytocannabinoids in cannabis inhibit metabolism of endocannabinoids by CYPs.
Thanks to our Guest Speakers for Spring 2018
January 11, 2018
Thank you, Natalie M. Johnson!
Professor of Immunology & Microbiology
Department of Environmental & Occupational Health
Texas A&M University School of Public Health
January 25, 2018
Thank you, Ronald Klein!
Department of Pharmacology, Toxicology & Neuroscience
Louisiana State University Health Sciences Center-Shreveport
February 8, 2018
Thank you, Michael F. Salvatore!
Institute for Healthy Aging, Center for Neuroscience Discovery
University of North Texas Health Science Center
February 22, 2018
Thank you, Judith T. Zelikoff!
Laboratory of Pulmonary & Systemic Toxicology
New York University School of Medicine
March 8, 2018
Thank you, Karen P. Maruska!
Department of Biological Sciences
Louisiana State University
March 22, 2018
Thank you, Mimi Sammarco!
Department of Surgery
Tulane University, New Orleans
April 5, 2018
Thank you, Jessy Satyadas Deshane!
Medicine/Division of Pulmonary, Allergy
& Critical Care Medicine
University of Alabama - Birmingham
April 19, 2018
Thank you, Claudia Jakubzick!
Department of Pediatrics, National Jewish Health
Department of Microbiology and Immunology
University of Colorado School of Medicine
May 3, 2018
Thank you, James Luyendyk!
Pathology and Diagnostic Investigation
Food Safety Toxicology
Michigan State University