James Reed

Assistant Research Professor, Department of Pharmacology, Louisiana State University Health Sciences Center, New Orleans, LA 70112

LSU Superfund Research Program Project 4 co-leader - Pollutant-Particle Systems and Xenobiotc Bioactivation

 

 

 

Contact:

Phone: (504) 568-5552

Fax: (504) 568-6888

Email: rreed@lsuhsc.edu

Website: http://www.medschool.lsuhsc.edu/pharmacology/reed.aspx

 

Research Interests, Expertise:

Academic and industrial experience studying metabolism of xenobiotics by diverse isoforms of cytochromes P450.  Recent work has focused on understanding how the aggregation and physical interactions of these enzymes and heme oxygenase-1 influence electron transfer from their common redox partner, the cytochrome P450 reductase.

 

Selected Publications:

2015

Reed, J.R., Cruz, A.L., Lomnicki, S.M., Backes, W.L. 2015. Inhibition of cytochrome P450 2B4 by Environmentally Persistent Free Radical-Containing Particulate Matter. Biochem Pharmacol. pii: S0006-2952 (15) 00173-2. Doi: 10.1016/j.bcp.2015.03.012. PMCID:
PMC4406862

Reed, J. R., Cruz, A. L. N. d., Lomnicki, S. M., & Backes, W. L. 2015. Inhibition of cytochrome P450 2B4 by environmentally persistent free radical-containing particulate matter. Biochemical Pharmacology. 95(2), 126-132. doi:http://dx.doi.org/10.1016/j.bcp.2015.03.012

 

2014 

Johnson, E.F., Connick, J.P., Reed, J.R., Backes, W.L., Desai, M.C., Xu, L., Estrada, R., Laurence, J.S., and Scott, E.E. 2014. Correlating Structure and Function of Drug-Metabolizing Enzymes: Progress and Ongoing Challenges. Drug Metabolism and Disposition. 42 (1): 9-22. Doi: 10.1124/dmd.113.054627. PMCID: PMC3876788

 

Reed, J.R., Cawley, G.F., Ardoin, T.G., Dellinger, B., Lomnicki, S.M., Hasan, F., Kiruri, L.W.,  Backes, W.L. 2014. Environmentally Persistent Free Radicals Inhibit Cytochromes P450 Activity in Rat Liver Microsomes. Toxicol Appl Pharmacol. Doi: 10.1016/j.taap.2014.03.021. PMCID:PMC4049543

 

2013

Reed, J.R., Cawley, G.F. and Backes, W.L. 2013. Interactions between cytochromes P450 2B4 (CYP2B4) and 1A2 (CYP1A2) lead to alterations in toluene disposition and P450 uncoupling. Biochemistry. 52(23):4003-4013. Doi:10.1021/bi400422a. PMCID: PMC3750074 

 

2012

Reed, J.R. and Backes, W.L. 2012. Formation of P450•P450 Complexes and Their Effect on P450 Function. Pharmacol Ther. 133(3):299-310. Doi: 10.1016/j.pharmthera.2011.11.009. PMCID:PMC3272114.

 

Reed, J.R., Connick, J.P., Cheng, D., Cawley, G.F., and Backes, W.L. 2012. Effect of Homomeric P450•P450 Complexes on P450 Function. Biochem J. 446(3):489-97. Doi: 10.1042/BJ20120636. PMCID:PMC3518449.

 

2011

Reed, J.R., Cawley, G.F., and Backes, W.L. 2011. Assessing an Antioxidant Role for HO-1 in the Inhibition of P450-Mediated Production of Reactive Oxygen Species in Rat Liver Microsomes. Drug Metabolism Letters. 5, 6-16.

 

Brignac-Huber, L., Reed, J.R., and Backes, W.L. 2011. Organization of NADPH-Cytochrome P450 Reductase and CYP1A2 in the Endoplasmic Reticulum – Microdomain localization affects monooxygenase function. Mol. Pharmacol. 79, 549-557.

 

Marohnic, C.C., Huber, W.J. III, Connick, J.P., Reed, J.R., McCammon, K., Panda, S.P., Martásek, P., Backes, W.L., and Masters, B.S.S. 2011. Mutations of Human Cytochrome P450 Reductase Differentially Modulate Heme Oxygenase-I Activity and Oligomerization. Arch. Biochem. Biophys. (in press).

 

2010

Reed, J.R., Eyer, M., and Backes, W.L.  2010. Functional Interactions between Cytochromes P450 1A2 and 2B4 Require Both Enzymes to Reside in the Same Phospholipid Vesicle: Evidence for physical complex formation. J. Biol. Chem. 285, 8942-8952. 

 

Reed, J.R., Huber, W.J., and Backes, W.L. 2010. Human Heme Oxygenase-1 Efficiently Catabolizes Heme in the Absence of Biliverdin Reductase. Drug Metab. Dispos. 38, 2060-2066. 

 

Reed, J.R.; Cawley, G.F.; and Backes, W.L. (2010). Inhibition of cytochrome P450 1A2-mediated metabolism and production of reactive oxygen species by heme oxygenase-1 in rat liver microsomes. Drug Metab. Lett. 5(1):6-16