Zeiss Light Sheet 7

Light Sheet Fluorescence Microscopy (LSFM) is an extremely powerful alternative to established fluorescence imaging techniques, especially for 3-D imaging within whole live organisms and large tissue explants. Ideal for fast and gentle imaging of whole living model organisms, tissues, and cells as they develop – over extended periods of time capable of imaging large optically cleared specimens in toto – with subcellular resolution. By selectively illuminating the observed optical section with a thin sheet of light, photo bleaching is reduced to a minimum, making light sheet microscopy ideal for nondestructive imaging of fragile samples over extended periods of time. Equipped with an incubation system with temperature and CO2 control for long-term in vivo experiments. 
Zeiss Lightsheet 7

Features and Specifications

Objectives

Magnification Objective Type  Description  NA Immersion WD (mm)
2.5 Fluar  2.5x 0.12

Clearing

8.7
5 EC Plan-Neofluar  5x 0.16 foc Water/Clearing 18.5
5 EC Plan-Neofluar  5x 0.16 Clearing 18.5
10 W Plan-Apochromat 

10x

0.50 Water 3.7
20 W Plan-Apochromat  20x 1.00 Water 2.4
20 W Plan-Apochromat  20x 1.00 Clearing 6.4

 

Lasers lines wavelengths (nm)

405, 445, 488, 514, 561, 638

Camera 

Two liquid cooled sCMOS cameras, pco.EDGE 

Filter Sets

Fluorochromes  Mirror  Filter 1 (nm)  Filter 2 (nm) 
DAPI/GFP  SBS LP 490   BP 420-470 (DAPI)  BP 505-545 (GFP) 
DAPI/RFP  SBS LP 510  BP 420-470 (DAPI)   BP 505-545 (GFP) 
GFP/mCherry  SBS LP 560  BP 505-545 (GFP)   LP 585 (mCherry) 
GFP/ Draq5(toto3)  SBS LP 560  BP 505-545 (GFP)  LP 660 (Draq5(toto3)) 
RFP/ Draq5(toto3)  SBS LP 640  BP 575-615 (RFP)   LP 660 (Draq5(toto3)) 

 

Available Capillary Tubes

Color Diameter (mm)
Red 0.68 
Black 1.00
Green 1.50
Blue 2.15