Area of Interest

I'm always excited to understand the relationships between microorganisms and biogeochemical cycles, particularly in marine systems. The lab takes a two-pronged approach to understanding these relationships: 1. Using cultivation independent techniques (e.g., metagenomics, metatranscriptomics, and single-cell genomics) to make observations about a system and develop hypotheses about which organisms are doing what, and 2. cultivation of microorganisms using a variety of methods to try and obtain the bugs identified by cultivation independent techniques as being numerically dominant, capable of important metabolisms, highly active, or containing genes of interest. 
 
The lab uses modern high-througput, dilution-to-extinction culturing techniques and maintains a culture collection established from source waters in the region. Work is ongoing in coastal southern Louisiana and the northern Gulf of Mexico "dead zone", and the lab is also involved with projects investigating the genomic basis for deep ocean SAR11 subclade differentiation.
 

Selected Publications

Schloss PD, Girard RA, Martin T, Edwards J, Thrash JC. (2016) Status of the Archaeal and Bacterial Census: an Update. mBio. 7(3).

Henson MW, Pitre DM, Weckhorst JL, Lanclos VC, Webber AT, Thrash JC. (2016) Correction for Henson et al., Artificial Seawater Media Facilitate Cultivating Members of the Microbial Majority from the Gulf of Mexico. mSphere. 1(3).

Henson MW, Pitre DM, Weckhorst JL, Lanclos VC, Webber AT, Thrash JC. (2016) Artificial Seawater Media Facilitate Cultivating Members of the Microbial Majority from the Gulf of Mexico. mSphere. 1(2).

Hogle SL, Thrash JC, Dupont CL, Barbeau KA. (2016) Trace Metal Acquisition by Marine Heterotrophic Bacterioplankton with Contrasting Trophic Strategies. Applied and environmental microbiology. 82(5):1613-24.

Learman DR, Henson MW, Thrash JC, Temperton B, Brannock PM, Santos SR, Mahon AR, Halanych KM. (2016) Biogeochemical and Microbial Variation across 5500 km of Antarctic Surface Sediment Implicates Organic Matter as a Driver of Benthic Community Structure. Frontiers in microbiology. 7:284.

Gillies LE, Thrash JC, deRada S, Rabalais NN, Mason OU. (2015) Archaeal enrichment in the hypoxic zone in the northern Gulf of Mexico. Environmental microbiology. 17(10):3847-56.

Carini P, Van Mooy BA, Thrash JC, White A, Zhao Y, Campbell EO, Fredricks HF, Giovannoni SJ. (2015) SAR11 lipid renovation in response to phosphate starvation. Proceedings of the National Academy of Sciences of the United States of America. 112(25):7767-72.

Carini P, Campbell EO, Morré J, Sañudo-Wilhelmy SA, Thrash JC, Bennett SE, Temperton B, Begley T, Giovannoni SJ. Discovery of a SAR11 growth requirement for thiamin's pyrimidine precursor and its distribution in the Sargasso Sea. (2014) The ISME journal. 8(8):1727-38.

Thrash JC, Temperton B, Swan BK, Landry ZC, Woyke T, DeLong EF, Stepanauskas R, Giovannoni SJ. (2014) Single-cell enabled comparative genomics of a deep ocean SAR11 bathytype.The ISME journal. 8(7):1440-51.

Smith DP, Thrash JC, Nicora CD, Lipton MS, Burnum-Johnson KE, Carini P, Smith RD, Giovannoni SJ. (2013) Proteomic and transcriptomic analyses of "Candidatus Pelagibacter ubique" describe the first PII-independent response to nitrogen limitation in a free-living Alphaproteobacterium. mBio. 4(6):e00133-12.