Area of Interest

My primary research interest is the elucidation of the structure and function of the proteins associated with Photosystem II in higher plants and cyanobacteria. We are particularly interested in examining the interactions between the intrinsic components (CP47, CP43, D1, D2, cytochrome b559 and a number of other low molecular mass peptides) and the extrinsic components (33, 24, and 17kDa) of this membrane protein complex. My group is utilizing site-specific labeling, protein cross-linking and site-directed mutagenesis to investigate these associations. Recently, we have demonstrated that the three extrinsic proteins associated with Photosystem II are required for photoautotrophy and photosystem assembly and stability in Arabidopsis. We are also interested in the identification of novel genes which are required for photosystem assembly and optimal photoautotrophy in the cyanobacterium Synechocystis 6803.
 

Selected Publications

Juneau, A., Frankel, L.K. Bricker, T.M. and Roose, J.L., “N-terminal Modification of CyanoQ in Synechocystis sp. 6803 is required for Protein Stability and Accumulation”. In Revision, PlosOne.

Mummadisetti, M.P., Frankel, L.K., Bellamy, H., Sallans, L., Goettert, J.S., Brylinski, M., and Bricker, T.M. “Use of Protein Crosslinking and Radiolytic Labeling to Elucidate the Structure of PsbO within Higher Plant Photosystem II”. Biochemistry 55,3204-3213 (2016).

Roose, J.L., Frankel, L.K. Mummadisetti, M.P. and Bricker, T.M., "The Extrinsic Proteins of Photosystem II: Update". Planta 243,889-908 (2016).

Virdi, K.S., Wamboldt, Y., Laurie, J.D., Kumar, K. R, Kundariya, H., Shao, M.-R., Leubker, S., Xu, Y., Elowsky, C., Day, P., Bricker, T.M., Elthon, T., Keren, I. and Mackenzie, S.A. “MutS HOMOLOG 1 in Plants Localizes to Sensory Plastids and Triggers Retrograde Signaling for Heritable Non-Genetic Changes in Development”. Molecular Plant, 9, 245-260 (2016).

Bricker, T.M, Mummadisetti, M.P., and Frankel, L.K. “Recent Advances in the use of Tandem Mass Spectrometry to Examine Structure/Function Relationships in Photosystem II”. Journal of Photochemistry and Photobiology B: Biology 152, 227-246 (2015).

Bell, A., Frankel, L.K.and Bricker, T.M., “High Yield Non-Detergent Isolation of Photosystem I-Light-Harvesting Chlorophyll II Membranes from Spinach Thylakoids: Implications for the Organization of the PS I Antennae in Higher Plants” Journal of Biological Chemistry 290, 18429-18437 (2015).

Mummadisetti, M.P., Frankel, L.K., Bellamy, H., Sallans, L., Goettert, J.S., Brylinski, M., Limbach, P.A. and Bricker, T.M. “Use of Protein Crosslinking and Radiolytic Labeling to Elucidate PsbP and PsbQ Interactions within Photosystem II of Higher Plants”. Proceedings of the National Academy of Sciences (USA) 111,16178-16183 (2014).

Zhang, P., Frankel, L.K. and Bricker, T.M., “Integration of apo-a-phycocyanin into phycobilisomes and its association with FNRL in the absence of the phycocyanin a-subunit lyase (CpcF) in Synechocystis sp. PCC 6803”. Plos One 9,e105952 (2014).

Roose, J.L., Frankel, L.K. and Bricker, T.M. “The PsbP-Domain Protein 1 Functions in the Assembly of Lumenal Domains in Photosystem I”. Journal of Biological Chemistry 289,23776-23785 (2014).

Bricker, T.M., Bell, A.J., Tran, L., Frankel, L.K. and Theg, S.M. “Photoheterotrophic Growth of Physcomitrella patens”. Planta 239,605-613 (2014).

Frankel, L.K., Sallans, L., Bellamy, H., Goettert, J.S., Limbach, P.A. and Bricker, T.M.“Radiolytic Mapping of Solvent Contact Surfaces in Photosystem II of Higher Plants: Experimental Identification of Putative Water Channels within the Photosystem”. Journal of Biological Chemistry 288, 23565-23572 (2013) Cover article.

Bricker, T.M., Roose, J.L., Zhang, P., Frankel, L.K. “The PsbP Family of Proteins”. Photosynthesis Research 116,235-250 (2013).

Frankel, L.K., Sallans, L., Limbach, P.A. and Bricker, T.M. “Oxidized Amino Acid Residues in the Vicinity of QA and PheoD1 of the Photosystem II Reaction Center: Putative Generation Sites of Reducing-Side Reactive Oxygen Species”. PLOS One 8,e58042 (2013).

Frankel, L.K., Sallans, L., Limbach, P.A. and Bricker, T.M. “Identification of Oxidized Amino Acid Residues in the Vicinity of the Mn4CaO5 Cluster of Photosystem II: Implications for the Identification of Oxygen Channels within the Photosystem”. Biochemistry, 51,6371-6377 (2012).

Burch, B., Bricker, T.M. and Putnam-Evans, C. “Mutations in the CP43 Protein of Photosystem II Affect PS II Function and Cytochrome C550 Binding”. In Artificial Photosynthesis (Najafpour, ed.), pp. 53-78, Intech (2012).

Bricker, T.M., Roose, J.L., Fagerlund, R.D., Frankel, L.K. and Eaton –Rye, J.J. “The Extrinsic Proteins of Photosystem II”. Biochimica et Biophysica Acta 1817,121-142 (2012).

Zhang, S., Frankel, L.K., and Bricker, T.M. "The Sll0606 Protein is Required for Photosystem II Assembly/Stability in the Cyanobacterium Synechocystis sp. PCC 6803". Journal of Biological Chemistry, Web published 8/20/10.

Roose, J.R., Frankel, L.K., and Bricker, T.M."Documentation of Significant Electron Transport Defects on the Reducing Side of Photosystem II upon Removal of the PsbP and PsbQ Extrinsic Proteins". Biochemistry 49, 36­41 (2010).

Liu, H., Frankel, L.K., and Bricker, T.M., "Characterization and Complementation of a psbR Mutant in Arabidopsis thaliana". Archives of Biochemistry and Biophysics 489,34-40 (2009).

Liu, H., Frankel, L.K. and Bricker, T.M.,"Functional Complementation of the psbo1 Mutant with a His6-Tagged PsbO-1 Protein in Photosystem II of Arabidopsis". Biochimica et Biophysica Acta 1787,1029-1038 (2009).

Yi, X., Hargett, S.R., Frankel, L.K. and Bricker, T.M., "The PsbP Protein, but not the PsbQ Protein, Is Required for Normal Thylakoid Architecture In Arabidopsis thaliana". FEBS Letters 583,2142-2147 (2009).

Zhang, S., Spann, K.W., Moroney, J.V. Frankel, L.K. and Bricker, T.M.,"The Involvement of Two Novel Genes, sll0804 and slr1306, in the CO2-concentrating Mechanism in the Cyanobacterium Synechocystis sp. strain PCC 6803". Journal of Bacteriology 190,8234-8237 (2008).

Yi, X., Hargett, S.R., Frankel, L.K. and Bricker, T.M., "The Effects of Simultaneous RNAi Suppression of PsbO and PsbP Protein Expression in Photosystem II of Arabidopsis". Photosynthesis Research 98,439-448 (2008).

Bricker, T.M. and Frankel, L.K.,"The Arabidopsis psbo1 Mutant is Cannot Efficiently Utilize Calcium in Support of Photosynthetic Oxygen Evolution". Journal of Biological Chemistry 283,29022-29027 (2008).

Yi, X., Liu, H., Hargett, S., Frankel, L.K., and Bricker, T.M.,"The PsbP Protein is Required for Photosystem II Complex Assembly/Stability and Photoautotrophy in Arabidopsis thaliana". Journal of Biological Chemistry 34,24833-24841 (2007).

Liu, H., Frankel, L.K. and Bricker, T.M., "Functional Analysis of Photosystem II in a PsbO-1 Deficient Mutant in Arabidopsis thaliana". Biochemistry 46,7607-7613 (2007).

Mason, C.B., Bricker, T.M., and Moroney, J.V.,"A rapid method for chloroplast isolation from the green alga Chlamydomonas reinhardtii". Nature-Protocols 1,2227-2230 (2006). Cover Article.

Lee, J., Bricker, T.M., LeFevre, M.P., Shannon R.M. and Oard, J.H. "Proteomic and genetic approaches to identifying defense-related proteins in rice challenged with the fungal pathogen Rhizoctonia solani". Molecular Plant Pathology" 7,405-416 (2006).

Yi, X., Hargett, S.R., Frankel, L.K. and Bricker, T.M., "The PsbQ Protein is Required for Arabidopsis Photosystem II Assembly/Stability and Photoautotrophy under Low Light Conditions". Journal of Biological Chemistry 281,26260-26267 (2006).

Bricker, T.M., "Commentary: A Time Resolved Vibrational Spectroscopy Glimpse of the Oxygen-Evolving Complex of Photosystem II". Proceedings of the National Academy of Sciences (USA) 103,7205-7206 (2006).

Bricker, T.M. and Burnap, R., "The Extrinsic Proteins of Photosystem II." In Photosystem II: The Light-Driven Water: Plastoquinone Oxidoreductase (Wydrzynski, T. and Satoh, K., eds), pp. 95-120, Springer (2005).

Meades, Jr., G.D., McLachlan, A., Sallans, L., Limbach, P.A., and Bricker, T.M. "Association of the 17 kDa Extrinsic Protein with Photosystem II in Higher Plants" Biochemistry 44,15216-15221 (2005).